|
KMID : 0383820100690040250
|
|
Tuberculosis and Respiratory Diseases
2010 Volume.69 No. 4 p.250 ~ p.255
|
|
|
Usefulness of Real-time PCR to Detect Mycobacterium tuberculosis and Nontuberculous Mycobacteria
|
|
Yun Eun-Young
Cho Su-Hee
Go Se-Il
Baek Jong-Ha
Kim Yu-Eun
Ma Jeong-Eun
Lee Gi-Dong
Cho Yu-Ji
Jeong Yi-Yeong
Kim Ho-Cheol
Lee Jong-Deog
Kim Sun-Joo
Hwang Young-Sil
|
|
|
Abstract
|
|
|
Background The purpose of this study was to evaluate recently developed real-time polymerase chain reaction (PCR) assay kit to detect Mycobacterium tuberculosis (MTB) and nontuberculous mycobacteria (NTM) in respiratory specimens.
Methods We assessed the positive rate of the real-time PCR assay to detect MTB and NTM in 87 culture-positive specimens (37 sputum, 50 bronchial washing), which were performed real-time PCR by using Real-Q™ MTB&NTM Kit from January 2009 to June 2009, at Gyeongsang University Hospital. To compare the efficacy with the TB-PCR assay, we evaluated 63 culture-positive specimens (19 sputum, 44 bronchial washing) for MTB or NTM, which were performed TB-PCR by using ABSOLUTE™ MTB II PCR Kit from March 2008 to August 2008.
Results Among 87 specimens tested using real-time PCR, MTB and NTM were cultured in 58 and 29, respectively. The positive rate of real-time PCR assay to detect MTB was 71% (22/31) and 92.6% (25/27) in AFB stain-negative and stain-positive specimens. For NTM, the positive rate of real-time PCR was 11.1% (2/18) and 72.7% (8/11) in AFB stain-negative and stain-positive specimens. Among 63 specimens performed using TB-PCR, MTB and NTM were cultured in 46 and 17, respectively. The positive rate of TB-PCR was 61.7% (21/34) and 100% (12/12) in AFB stain-negative and stain-positive specimens. TB-PCR was negative in all NTM-cultured 17 specimens.
Conclusion TB/NTM real-time PCR assay is useful to differentiate MTB and NTM in AFB stain-positive respiratory specimens and it is as effective in detecting MTB with TB-PCR.
|
|
|
KEYWORD
|
|
|
Mycobacterium tuberculosis, Mycobacteria, Atypical, Polymerase Chain Reaction, Real-Time
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
  
|
|